The Development And Analysis Of Microsatellite Dna Essay

10 THE DEVELOPMENT AND ANALYSIS OF MICROSATELLITE DNA Summary Due to the significant negative impact of the industrial activities on the habitat, many species have become endangered. To conserve the species, a thorough investigation is needed. In this case, the research is based on the study the interdependencies between the genotype and spatial dispersal of populations. Despite being endangered, turtles comprise rather specific object for such investigations, as well as any other long-lived organisms, because their reproduction cycles complicate the genetic investigations using conventional methods. That is why the novel DNA markers, microsatellites, were chosen for this research. Although the microsatellites are very informative and universal, the overall cost for research is higher than for RAPD and AFLP. This is mostly due to the cost of the fluorescent dye which is used in the PCR. To eliminate this, I will apply an interesting technique which allows for incorporation of the dye by the forward primer into the product of polymerization thus saving a considerable amount of the dye. Generally, I plan to base research on such steps: - Studying the populations of Apalone spinifera and Apalone mutica based on available data; - Taking samples of blood from the animals which are going to be caught by hand in their usual habitat. The sampling will occur in the least possible harmful way; - Testing 12 pairs of primers initially developed for Apalone spinifera using theShow MoreRelatedGenetic Viological Markers, Cytological Markers And Biochemical Markerss?908 Words   |  4 PagesIntroduction The traditional genetic analysis within populations by morphological markers, cytological markers and biochemical markers has not been very efficient when the selection objective involves several characteristics with unfavorable genetic correlation (Schwerin et al., 1995). 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